UterineAdeTM

 

Composition:
Each 100 cc contains 20 grams of mannose and other monosaccharide sugars; 0.85 grams of sodium chloride, natural trace essential minerals from sea salts, buffered with sodium salts of organic acids and purified water.

Action:
The principle of this monosaccharide solution is based on research which showed normal bacterial flora utilized monosaccharide metabolites to naturally inhibit pathogenic bacteria of the skin and mucous membranes1. Further research demonstrated that these monosaccharide sugars and their metabolites may be used to inhibit common pathogenic bacteria and fungi of the reproductive tract of mares. Research by other workers has confirmed that specific sugars can indeed prevent pathogenic bacteria from infecting mucosal and epithelial cells of the reproductive tract of mares 2. Also, it has been proven that that adequate sodium is essential for optimum reproductive fertility. An increase in sodium in the cervico-vaginal mucus is required during estrus for high conception rates3. A deficiency in sodium increases infertility and embryonic mortality 4,5. UterineAde solutions contain natural sodium sea salts.

Indications:
For post foaling or aborting infected mares, the product may be used with other fluids and antibiotics. For the most efficient use of the product, other fluids such as saline lavage solutions may be used to remove debris from the uterus. After the debris and fluid are removed, infuse the uterus with undiluted UterineAde. UterineAde will promote the contraction of the uterus and will aid in clearing the uterus of excessive fluid. Use UterineAde daily until the uterus returns to normal.

Dosage and administration:
For post-foaling and infected mares, 100 to 500 cc intrauterine, undiluted, per day, repeat daily until infection clears. For mares to be bred, 60 to 100 cc per day on the first day of estrus, repeat daily if necessary. Do not use the day of breeding. May be used 24 to 48 hours post-breeding.

This protocol will aid in the prevention of venereal bacterial infections. This product has been formulated to be used without antibiotics. However, systemic antibiotics and other conventional treatments are recommended in addition to UterineAde if post foaling or aborting mares are infected with pathogenic microorganisms to aid in the prevention of septicemia and laminitis.

Although UterineAde can be used without antibiotics, it can also be used in conjunction with antibiotics to aid in the healing of lesions while neutralizing microbial organisms. UterineAde will neutralize the microbial organisms if the bacteria are resistant to the antibiotics and may also allow antibiotics to be more effective. UterineAde not only will neutralize pathogens, but has the added benefit of healing lesions in the uterine mucosa.

Caution:
For veterinary use only. Not to be used on animals intended for food. Keep out of the reach of children.
Side effects: There are no known side effects.

UterineAde may be used in combination with antibiotics to treat and prevent antibiotic resistant bacterial infections in mares and stallions.

UterineAde was originally developed to treat and prevent bacterial infections caused by Taylorella equigenitalis, the causative organism for contagious equine metritis.  The overuse of antibiotics and potent disinfectants to treat T. equigenitalis,and similar pathogens caused bacteria to become antibiotic resistant leading to super bacterial infections.  Seemingly, when bacteria become resistant to antibiotics, they are more pathogenic and induce severe diseases of various tissues and organs including the reproductive tract.  Mares carrying these antibiotic resistant bacteria are not only more difficult to get pregnant, but may also spread venereal bacteria to stallions.  Then these antibiotic resistant venereal bacteria may spread to other mares.

UterineAde, containing monosaccharide antibacterial and antifungal metabolites, naturally inhibits pathogenic bacteria without affecting the normal flora.  And, these natural antibacterial metabolites will not cause antibiotic resistance and super bacterial infections.

H. Libor DVM in the Check Republic first demonstrated that UterineAde will change antibiotic resistant bacteria to become sensitive to commonly used antibiotics.  Other veterinarians and local equine practitioners later confirmed these findings.

Mares infected with various antibiotic resistant bacteria, and failing to conceive while infected with these bacteria, Dr. Libor found that after treating these mares with UterineAde for up to 4 days, the antibiotic resistant pathogenic bacteria present in the reproductive tract then became sensitive to commonly used antibiotics, like penicillin and gentamicin.  Then after short cycling these mares, they were treated with UterineAde in combination with an antibiotic, like penicillin, before and after breeding.  This treatment was successful in the mares becoming pregnant.

These results suggest that UterineAde may be used to enhance the activity of many antibiotics, especially for bacterial infections that are resistant to antibiotics.

Antibiotic sensitive discs with and without UterineAde.  The bacteria in the Petri dish are methicillin-resistant Staphylococcus aureus.

Disc 1, blank disc.  Disc 2, penicillin.   Disc 3, ampicillin. Disc 4, UterineAde.   Disc   5, penicillin and UterineAde.  Disc 6, ampicillin and UterineAde.   Disc 4, 5 and 6 have the same concentration of UterineAde

Note:  The combination of antibiotics with UterineAde allows the bacteria to become sensitive to antibiotics.  Also, antibiotics are enhanced when combined with UterineAde.  The bacteria around discs 2 and 3 are resistant to antibiotics without UterineAde.

References:



1. Swerczek, T.W.: Inhibition of the CEM organism by the Normal Flora of the Reproductive Tract. Vet. Rec. 1978, 103:125.

2. King, S.S. et.al.: Use of specific sugars to inhibit bacterial adherence to equine endometrium in vitro. AJVR. 2000, 61:446-449

3. Arya, S.P. and Jain, Y.C.: 1986. Sodium and potassium concentrations of cervico-vaginal mucus in relation to oestrous cycle and early pregnancy 
in Jersey cows. Indian Journal of Animal Science. 56:331.

4. Harris, D.J., Allen, J.D., and Caple, I.W.: 1986. Effects of low sodium nutrition on fertility of dairy cows. Proceeding of the 
Nutritional Society of Australia 11:92.

5. Cromwell, G.L., Hall, D.D. Combs, O.M. et.al.: 1989. Effects of dietary salt level during gestation and lactation on reproductive 
performance of sows. A cooperative study. J. Animal Science. 67:374.

Mechanism of action for intrauterine sugar solutions

Scientific Literature

Monosaccharide sugars have been shown to be effective in inhibiting bacteria that commonly infect the genital-urinary tract of mares. One of the benefits of sugars is that they can be used alone or with antibiotics to treat uterine infections in mares. Specific sugars can inhibit bacterial adherence to the equine endometrium in vitro. Mannose and N-acetyl-D-galactosamine inhibited adhesion of Escherichia coli and Pseudomonas aeruginosa to epithelial cells, whereas only mannose inhibited adhesion of Streptococcus zooepidemicus. In horses with uterine infections, use of sugars to competitively displace bacteria from attachment sites on cells may provide an adjunct to antibiotic treatment 1.

Bacterial adherence to cell surfaces and phagocytosis of bacteria is important as it relates to pathogenicity of various strains of bacteria which are common causes of uterine infections including Escherichia coli, Pseudomonas aruginosia, Streptococcus zooepidemicus, Salmonella spp., and other bacteria. The adherence of bacteria is inhibited by sugars such as L-fructose and D-galactose, which suggest that sugar-mediated adherence is widespread. The intercellular recognition is thought to be mediated by sugar residues such a D-mannose on the surface of cells to which bacteria attach by sugar-binding substance of their surface. The nature of the receptors is unknown, but there is evidence that bacteria, like E. coli produces lectin-like substances specific for D-mannose, by which it binds to the cells 2.

  1. King SS, Young DA, Nequin LG, Carnevale EM. Use of specific sugars to inhibit bacterial adherence to equine endometrium in vitro. Am J Vet Res. 2000 ;61:446-9.
  2. Sharon N, Eshdat Y, Silverblatt FJ, Ofek I. Bacterial adherence to cell surface sugars. Ciba Found Symp. 1981;80:119-41.